Sampling Manual: How to Collect Air Samples for Airborne Mould Spores

LCS Laboratory Inc., based in London, Ontario, provides mould sampling kits and laboratory analysis for assessing airborne mould spores in indoor environments. These kits are suitable for homeowners, indoor air quality consultants, and building professionals who need a practical way to evaluate air quality.

When Should You Test for Mould?

Air sampling for mould spores may be useful when there are signs or conditions that support microbial growth, such as moisture intrusion, past water damage, musty odours, or visible mould. Testing can help document indoor air conditions and support further investigation if needed.

What’s Included in the Sampling Kit?

Each mould sampling kit includes the basic equipment required to collect air samples:

  • Portable sampling pump for controlled airflow.
  • Single-use Air-O-Cell® cassettes designed to capture mould spores, pollen, and other airborne particles.
  • Step-by-step instructions for sample collection.

Planning Your Sampling

Careful planning helps ensure consistent and interpretable results:

  • Cassette use: Each cassette is single-use. Plan for one sample per room or area.
  • Sampling time: Individual samples typically take about 10 minutes. Most projects can be completed within 30 to 60 minutes.

Sampling Guidelines

Follow these general practices to improve sample quality:

  • Collect samples in regularly occupied areas.
  • Keep windows and exterior doors closed for approximately 24 hours before sampling, except for normal entry and exit.
  • Turn off fans or ventilation systems that bring in outdoor air.
  • Place the cassette near the centre of the room, at a height of 3 to 6 feet above the floor (breathing zone).

Outdoor Reference Sample

Outdoor air samples are often used as a reference point when interpreting indoor results. Collecting at least one outdoor sample, particularly during warmer months, can help provide useful context.

Additional Resources

For more detailed guidance on setting up the sampling equipment, refer to our article or watch this video from Zefon Inc.: Air-O-Cell sampling demonstration video

Ordering and Questions

Mould sampling kits can be ordered through our online store:


Order mould sampling kit

If you have questions about sampling or interpreting results, feel free to contact us. ©

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